Purpose
This kit allows for the determination of ATGL concentrations in Human serum,
blood plasma, and other biological fluids
.
Principle of the assay
T
he kit assay Human ATGL level in the sample
，
use Purified Human ATGL
antibody to coat microtiter plate wells, make solid-phase antibody, then add ATGL to
wells, Combined ATGL antibody which With HRP labeled
，
become antibody-antigen
enzyme-antibody complex, after washing Completely, Add TMB substrate solution,
TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated
by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm. The concentration of ATGL in the
samples is then determined by comparing the O.D. of the samples to the standard curve.
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